2010 Fiscal Year Final Research Report
Analysis of epigenetic regulation of cone opsin expression by COUP-TF nuclear receptors
| Project/Area Number |
21770136
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATOH Shinya The University of Tokyo, 医科学研究所, 特任助教 (80333558)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 網膜 / 核内受容体 / オプシン |
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| Research Abstract |
Previously we demonstrated that COUP-TF nuclear receptors contribute to the establishment of dorso-ventral expression pattern of cone opsins in mouse retina. In this study I'd like to clarify the mechanism of this regulation by focusing on epigenetic mechanism. I analyzed DNA methylation status of dorsal- and ventral-region of mouse retina, or DNA methylation and histone modification status in retinoblastoma cell line in which endogenous COUP-TFs were knocked down by shRNA. However, significant correlation between COUP-TFs expression and these epigenetic status has not been seen yet. On the other hand, I identified TRbeta2 regulating genomic region in human red opsin gene and found that COUP-TFs show the different effect on TRbeta2-mediated activation in this genomic region between human and mouse genome. Furthermore, I found that Brg1, a chromatin remodeling factor, activates blue opsin gene expression and COUP-TFs suppress this Brg1-mediated activation. These results suggest that COUP-TFs regulate cone opsin expression in retina through interaction of these factors, which establishes each animal specific cone opsin expression pattern.
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