2010 Fiscal Year Final Research Report
Studies on regulation of transport vesicle budding from endoplasmic reticulum by the calcium-binding protein ALG-2
| Project/Area Number |
21770139
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
SHIBATA Hideki 名古屋大学, 生命農学研究科, 准教授 (30314470)
|
|---|
| Research Collaborator |
MAKI Masatoshi 名古屋大学, 大学院・生命農学研究科, 教授 (40183610)
WADA Ikuo 福島県立医科大学, 医学部, 教授 (40182969)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | 細胞情報伝達機構 / 小胞輸送 / カルシウム / COPII 小胞 / タンパク質問相互作用 / 蛍光イメージング |
|---|
| Research Abstract |
Sec31A is an outer component of COPII coat of endoplasmic reticulum (ER)-to-Golgi transport vesicles. We previously found that the penta-EF-hand protein ALG-2 calcium-dependently interacts with Sec31A. In this study, we first narrowed the Sec31A region responsible for ALG-2 binding to 13 amino acids. Next, fluorescence recovery after photobreaching analysis revealed that the ALG-2 binding region constitutes a high affinity binding site of Sec31A for the ER membrane. Finally, the calcium/phospholipid binding protein annexin All was identified as a recruiting protein to the budding site of COPII by the adaptor function of ALG-2.
|
