2010 Fiscal Year Final Research Report
Identification of genes involved in transport of GPI-anchored proteins
| Project/Area Number |
21770140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
FUJITA Morihisa Osaka University, 微生物病研究所, 特任助教 (30532056)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 糖鎖生物学 / タンパク質輸送 |
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| Research Abstract |
Glycosylphosphatidylinositol (GPI) anchoring of proteins is a post-translational modification occurring in the endoplasmic reticulum (ER). Here, we established mutant cell lines that were selectively defective in transport of GPI-anchored proteins from the ER to the Golgi. We identified a responsible gene, designated PGAP5 (post-GPI-attachment to proteins 5). PGAP5 catalyzed the remodeling of the glycan moiety on GPI-APs. Our data demonstrate that GPI glycan acts as an ER-exit signal and suggest that glycan remodeling mediated by PGAP5 regulates GPI-AP transport in the early secretory pathway
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