Signaling pathway of Xenopus egg activation

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770142
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kobe University
• Principal Investigator: IWASAKI Tetsushi Kobe University, 自然科学系先端融合研究環遺伝子実験センター, 技術専門職員 (40379483)
• Project Period (FY): 2009 – 2010
• Keywords: 細胞情報伝達機構

Research Abstract

We have previously demonstrated that Src tyrosine kinase plays an important role in the Xenopus egg activation. However, the molecular mechanism of egg activation is still unclear. Here, we investigated the activation mechanism and the physiological function of Src kinase in Xenopus egg fertilization. Biochemical and immunochemical analyses have revealed the involvement of heterotrimeric G proteins in sperm-induced Src activation. Moreover, we analyzed hnRNP K protein, which is known as a substrate of Src kinase in Xenopus egg fertilization. Our molecular biological and biochemical analyses showed that the hnRNP K binds to specific mRNAs in unfertilized egg, and that Src-mediated tyrosine phosphorylation acts as a trigger for their dissociation at egg activation.

Research Products

• [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs (2010)
  • Author(s): Tokmakov A.A., Iwasaki T., Sato KI., Fukami Y.
  • Journal Title: Methods 51(1) Pages: 177-182
• [Journal Article] Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization. (2009)
  • Author(s): Mammadova G., Iwasaki T., Tokmakov A.A., Fukami, Y., Sato K.-I.
  • Journal Title: BMC Dev.Biol. 9 Pages: 68
• [Journal Article] TPA inhibits melanoma growth by inactivation of STAT3 through PKC-activated tyrosine phosphatase(s). (2009)
  • Author(s): Oka M., Sumita N., Sakaguchi M., Iwasaki T., Bito T., Kageshita T., Sato K.I., Fukami Y., Nishigori C.
  • Journal Title: J Biol Chem. 284(44) Pages: 30416-30423
• [Journal Article] Physiology and gene expression of the rice landrace Horkuch under salt stress
  • Author(s): Laisa A.Lisa, Sabrina M.Elias, M.Sazzadur Rahman, Saima Shahid, Tetsushi Iwasaki, A.K.M.Mahbub Hasan, Keiko Kosuge, Yasuo Fukami, Zeba I.Seraj
  • Journal Title: Functional Plant Biology 38(4) Pages: 282-292
• [Presentation] Evolutional and functional analysis of Xenopus STAT3 (2011)
  • Author(s): Tetsushi Iwasaki, Sho Iguchi, Alexander Tokmakov, Yasuo Fukami.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "The Evolution of Protein Phosphorylation"
  • Place of Presentation: Keystone, US
  • Year and Date: 2011-01-25
• [Presentation] Functional analysis of STAT3 during Xenopus oocyte maturation (2010)
  • Author(s): Tetsushi Iwasaki, Sho Iguchi, Takanori Hashimoto, Minori Sasakura, Alexander Tokmakov, Yasuo Fukami.
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Effect of Src kinase inhibition on melanoma cell growth (2010)
  • Author(s): Tomoyo Tani, Alexander Tokmakov, Tetsushi Iwasaki, Yasuo Fukami
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] FM-AFM Study of Proteins on Lipid Rafts (2010)
  • Author(s): T.Sugihara, T.Hiasa, H.Onishi, K.Kimura, M.Ohta, K.Watanabe, R.Kokawa, N.Oyabu, K.Kobayashi, H.Yamada, T.Iwasaki, Y.Fukami.
  • Organizer: The 12th International Scanning Probe Microscopy Conference
  • Place of Presentation: 札幌
  • Year and Date: 2010-05-10
• [Presentation] Involvement of phosphatidylinositol 3-kinase in signal transduction at fertilization in Xenopus eggs (2009)
  • Author(s): Gunay Mammadova, Tetsushi Iwasaki, Tokmakov A.Alexander, Yasuo Fukami, Ken-ichi Sato
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Expression analysis of hnRNP K-binding mRNAs during Xenopus early development (2009)
  • Author(s): Tetsushi Iwasaki, A.K.M.Mahbub Hasan, Sho Iguchi, Gunay Mammadova, Tokmakov A.Alexander, Ken-ichi Sato, Yasuo Fukami.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Unfertilized Xenopus eggs die by apoptosis after MAPK inactivation (2009)
  • Author(s): Tokmakov A, Alexander, Sho Iguchi, Tetsushi Iwasaki, Yasuo Fukami
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Analysis of Xenopus Src and G protein functions in egg activation (2009)
  • Author(s): A.K.M.Mahbub Hasan, Tetsushi Iwasaki, Gunay Mammadova, Sho Iguchi, Tokmakov A.Alexander, Ken-ich Sato, Yasuo Fukami
  • Organizer: 第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Involvement of Phosphatidylinositol 3-kinase in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization (2009)
  • Author(s): Gunay Mammadova, Shota Kushima, Tetsushi Iwasaki, Alexander A.Tokmakov, Yasuo Fukami, Ken-ichi Sato
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] Molecular mechanism of Src activation in Xenopus egg fertilization (2009)
  • Author(s): Tetsushi Iwasaki, A.K.M. Mahbub Hasan, Tokmakov A. Alexander, Gunay Mammadova, Ken-ichi Sato, Yasuo Fukami
  • Organizer: Gordon Research Conference "Phosphorylation & G-Protein Mediated Signaling Network"
  • Place of Presentation: Biddeford, US
  • Year and Date: 2009-06-10