2010 Fiscal Year Final Research Report
The roles of glycosphingolipids in Saccharomyces cerevisiae
| Project/Area Number |
21770148
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tohoku Pharmaceutical University |
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Principal Investigator |
UEMURA Satoshi Tohoku Pharmaceutical University, 薬学部, 助教 (10399975)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 糖鎖生物学 |
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| Research Abstract |
In this study, to clarify the involvement of glycosphingolipid (GSL) in dynamics of plasma membrane proteins, a series of mutants of the yeast Saccharomyces cerevisiae in GSLs biosynthesis was analyzed by fluorescence recovery after photobleaching (FRAP). FRAP is a technique to study protein mobility in living cells by measuring the rate of fluorescence recovery at a bleached region. By this analysis, I found that the mobile fraction of Hxt1-EGFP in Δsur2Δcsg1Δcsh1 mutant remarkably decreased. Moreover, the diffusion time of Hxt1-EGFP in the mutant was slow. These results indicate that both the extension of glycan moieties of GSL and the hydroxylation of C4-sphingoid long chain base are necessary to maintain the rate of lateral diffusion of Hxt1-EGFP at the plasma membrane.
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