2010 Fiscal Year Final Research Report

The roles of glycosphingolipids in Saccharomyces cerevisiae

Research Project

Project/Area Number	21770148
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Functional biochemistry
Research Institution	Tohoku Pharmaceutical University
Principal Investigator	UEMURA Satoshi Tohoku Pharmaceutical University, 薬学部, 助教 (10399975)
Project Period (FY)	2009 – 2010
Keywords	糖鎖生物学
Research Abstract	In this study, to clarify the involvement of glycosphingolipid (GSL) in dynamics of plasma membrane proteins, a series of mutants of the yeast Saccharomyces cerevisiae in GSLs biosynthesis was analyzed by fluorescence recovery after photobleaching (FRAP). FRAP is a technique to study protein mobility in living cells by measuring the rate of fluorescence recovery at a bleached region. By this analysis, I found that the mobile fraction of Hxt1-EGFP in Δsur2Δcsg1Δcsh1 mutant remarkably decreased. Moreover, the diffusion time of Hxt1-EGFP in the mutant was slow. These results indicate that both the extension of glycan moieties of GSL and the hydroxylation of C4-sphingoid long chain base are necessary to maintain the rate of lateral diffusion of Hxt1-EGFP at the plasma membrane.

(3 results)