Functional analysis of heparan sulfate fine structures in Drosophila axon guidance

2011 Fiscal Year Final Research Report

• Project/Area Number: 21770154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: 財団法人東京都医学総合研究所 (2011); Tokyo Metropolitan Organization for Medical Research (2009-2010)
• Principal Investigator: KAMIMURA Keisuke 財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 主席研究員 (30529524)
• Project Period (FY): 2009 – 2011
• Keywords: ヘパラン硫酸プロテオグリカン / ショウジョウバエ

Research Abstract

We have established the "glyocogenetics" approach, which allow the manipulation of heparan sulfate fine structures at will, using Drosophila, for which sophisticated genetic techniques are available. We also found that HSPGs have important roles in synapse formation.

Research Products

• [Journal Article] In vivo manipulation of heparan sulfate structure and its effect on Drosophila development (2011)
  • Author(s): Keisuke Kamimura, Nobuaki Maeda, and Hiroshi Nakato
  • Journal Title: Glycobiology Volume: 21(5) Pages: 607-618
  • DOI: doi:10.1093/glycob/cwq202
  • Peer Reviewed
• [Journal Article] Functions of chondroitin sulfate and heparan sulfate in the developing brain (2011)
  • Author(s): Nobuaki Maeda, Maki Ishi, Kazunari Nishimura, and Keisuke Kamimura
  • Journal Title: Neurochem Res Volume: 36(7) Pages: 1228-1240
  • DOI: doi:10.1007/s11064-010-0324-y
  • Peer Reviewed
• [Journal Article] Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation (2010)
  • Author(s): Adam Kleinschmit, Takashi Koyama, Katsufumi Dejima, Yoshiki Hayashi, Keisuke Kamimura and Hiroshi Nakato
  • Journal Title: Dev. Biol Volume: 345(2) Pages: 204-214
  • DOI: doi:10.1016/j.ydbio.2010.07.006
  • Peer Reviewed
• [Journal Article] Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization (2010)
  • Author(s): Kazunari Nishimura, Maki Ishii, Mutsuki Kuraoka, Keisuke Kamimura, and Nobuaki Maeda
  • Journal Title: Neuroscience Volume: 169(4) Pages: 1535-1547
  • DOI: doi:10.1016/j.neuroscience.2010.06.027
  • Peer Reviewed
• [Presentation] Perlecan regulates Wingless signaling at the Drosophila neuromuscular junction (2011)
  • Author(s): Keisuke Kamimura and Nobuaki Maeda
  • Organizer: 7^<th> International Conference on Proteoglycans
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2011-10-20
• [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはWntシグナルを調節する (2011)
  • Author(s): 神村圭亮、前田信明
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2011-09-24
• [Presentation] ショウジョウバエの神経筋接合部においてパールカンはWinglessシグナル伝達を調節する (2011)
  • Author(s): 神村圭亮、前田信明
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-09-15
• [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの役割 (2011)
  • Author(s): 神村圭亮、前田信明
  • Organizer: 第29回日本糖質学会大会
  • Place of Presentation: 岐阜県高山市
  • Year and Date: 2011-09-10
• [Presentation] Perlecan regulates BMP signaling essential for synaptic growth (2011)
  • Author(s): 神村圭亮、前田信明
  • Organizer: 第28回内藤コンファレンス「糖鎖の発現と制御[I]-機能から病態まで-」
  • Place of Presentation: 神奈川県湘南国際村センター
  • Year and Date: 2011-07-28
• [Presentation] In vivo manipulation of heparan sulfate structure and its effects on Drosophila development (2010)
  • Author(s): 神村圭亮、前田信明、中藤博志
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-09
• [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはBMPシグナルを調節する (2010)
  • Author(s): 神村圭亮、前田信明
  • Organizer: Neuro2010
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-03
• [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの機能解析 (2009)
  • Author(s): 神村圭亮、前田信明
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2009-10-24
• [Remarks]
  • URL: http://www.igakuken.or.jp/research/project/res_prj08.html