Neutron crystallographic analysis of human transthyretin

2011 Fiscal Year Final Research Report

• Project/Area Number: 21770159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: University of Toyama (2011); Ibaraki University (2009-2010)
• Principal Investigator: YOKOYAMA Takeshi 富山大学, 大学院・医学薬学研究部(薬学), 助教 (50524162)
• Project Period (FY): 2009 – 2011
• Keywords: 中性子結晶構造解析

Research Abstract

Towards to the neutron crytallographic analysis, crystallization of large crytals and neutron diffraction data collection were carried out, and we succeeded to determine the neutron crystal structure of transthyretin. Neutron structure revealed the structural stability and molecular pH sensitivity, related to the amyloid fibril formation.

Research Products

• [Journal Article] Hydrogen-bond network and pH sensitivity in transthyretin : Neutron crystal structure of human transthyretin (2012)
  • Author(s): Yokoyama T., Mizuguchi M., Nabeshima Y., Kusaka K., Yamada T., Hosoya T., Ohhara T., Kurihara K., Tomoyori K., Tanaka I., Niimura N.
  • Journal Title: J. Struct. Biol Volume: 177-2 Pages: 283-290
  • Peer Reviewed
• [Journal Article] Neutron structure analysis using the IBARAKI biological crystal diffractometer(iBIX) at J-PARC (2010)
  • Author(s): Tanaka I., Kusaka K., Hosoya T., Niimura N., Ohhara T., Kurihara K., Yamada T., Ohnishi Y., Tomoyori K., Yokoyama T.
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 66-11 Pages: 1194-1197
  • Peer Reviewed
• [Journal Article] Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme (2009)
  • Author(s): Yokoyama T., Choi KJ., Bosch AM., Yeo HJ.
  • Journal Title: Biochim Biophys Acta Volume: 1974-7 Pages: 1073-1081
  • Peer Reviewed
• [Journal Article] Structure of a sigma28-regulated nonflagellar virulence protein from Campylobacter jejuni (2009)
  • Author(s): Kirkpatrick AS., Yokoyama T., Choi KJ., Yeo HJ.
  • Journal Title: Biochem Biophys Res Commun Volume: 6 ; 380(2) Pages: 407-412
  • Peer Reviewed
• [Presentation] トランスサイレチンの結晶構造解析 (2012)
  • Author(s): 横山武司
  • Organizer: 第3回MLFシンポジウム
  • Place of Presentation: 東海(口頭発表)
  • Year and Date: 20120119-20
• [Presentation] XXII Congress and General Assembly International Union of Crystallography(IUCr2011) (2011)
  • Author(s): Yokoyama T., Nabeshima Y., Hosoya T., Ohhara T., Kurihara K., Kusaka K., Mizuguchi M., Tanaka I., Niimura N.
  • Place of Presentation: マドリッド(ポスター発表)
  • Year and Date: 20110822-29
• [Presentation] トランスサイレチンの結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 平成22年度茨城県中性子ビームライン成果報告会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2011-09-26
• [Presentation] トランスサイレチンの中性子結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 第12回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 富山(口頭発表)
  • Year and Date: 2011-06-18
• [Presentation] 変異体トランスサイレチンの中性子回折測定及びX線結晶構造解析 (2010)
  • Author(s): Yokoyama T., Nabeshima Y., Hosoya T., Ohhara T., Kurihara K., Kusaka K., Mizuguchi M., Tanaka I., Niimura N.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2010-09-20
• [Presentation] 変異体トランスサイレチンの中性子回折実験 (2010)
  • Author(s): 横山武司
  • Organizer: 水和ナノ構造研究会
  • Place of Presentation: 伊豆(口頭発表)
  • Year and Date: 2010-09-06
• [Presentation] Towards the neutron crystallographic analysis of amyloid protein, TTR. (2009)
  • Author(s): Yokoyama T., Nabeshima Y., Mizuguchi M., Tanaka I., Niimura N.
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島(ポスター発表)
  • Year and Date: 2009-10-30