2010 Fiscal Year Final Research Report
Investigation of translation regulation by quantification of mRNA and corresponding protein in living cells
| Project/Area Number |
21770162
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OKABE Kohki 東京大学, 大学院・薬学系研究科, 助教 (20455398)
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| Project Period (FY) |
2009 – 2010
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| Keywords | バイオイメージング / mRNA / 翻訳 |
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| Research Abstract |
In this study, the translation regulation was investigated by the quantitative imaging of endogenous mRNA and protein in living cells. By quantifying GFP mRNA and its corresponding protein, GFP in the same cell, I observed a considerable cell-to-cell variation in translation activity. Furthermore, the mRNA dynamics analyzed in stressed cells indicated that the translation repression was maintained by harboring endogenous mRNA in stress-induced granules.
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