2011 Fiscal Year Final Research Report
Structural analysis of novel type 6 secretion apparatus proteins
| Project/Area Number |
21770164
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KANAMARU Shuji 東京工業大学, 生命理工学研究科, 助教 (50376951)
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| Project Period (FY) |
2009 – 2011
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| Keywords | グラム陰性菌 / 分泌装置 / バクテリオファージ / 構造解析 / T6SS / βヘリックス / VgrG |
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| Research Abstract |
To determine the crystal structure of VgrG protein of Type 6 secresion apparatus from E. coli O157 and E. coli CFT073, over-expression vectors for both full-length and C-terminal domain of VgrG were constructed. We succeeded in establish the purification methods for VgrG from E. coli O157. Purified protein was treated with trypsin to get stable VgrG1^M467-his. This VgrG1^M467-his was examined thousands of crystallization condition. One of the conditions gave nice crystal formation for structure determination. Now we are trying get isomorphous heavy atom derivatives for phase determination. Moreover, we are going to establish other VgrG purification strategy.
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Small
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Journal Title
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Pages: 808-13
Peer Reviewed
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Author(s)
Leiman, P. G., Arisaka, F., van Raaij, M. J., Kostyuchenko, V. A., Aksyuk, A. A., Kanamaru, S. & Rossmann, M. G.
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Journal Title
Peer Reviewed
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