2010 Fiscal Year Final Research Report
Development of 2-step excitation transient grating method, and elucidation of photo-reaction mechanism and control of signal transferring protein.
| Project/Area Number |
21770165
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
INOUE Keiichi Nagoya Institute of Technology, 大学院・工学研究科, 助教 (90467001)
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| Research Collaborator |
SUDO Yuki 名古屋大学, 理学研究科, 准教授 (10452202)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 光受容タンパク質 / 過渡回折格子法 |
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| Research Abstract |
Sensory rhodopsin I from eubacterium Salinibacter ruber is the photo-censor for the bacterial photo-taxis. In this study the photo-reaction dynamics of this protein was studied by transient grating (TG) method. As the result, the presences of new photo-intermediates were identified in the photo-cycle of sensory rhodopsin I. In addition, the enthalpy differences of the intermediates were determined by TG method and we found that they are significantly affected by the binding and dissociation of chloride ion.
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