2010 Fiscal Year Final Research Report
Analysis of interaction, ion transport and structural change in a stator complex of bacterial flagellar motor
| Project/Area Number |
21770166
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
SUDO Yuki Nagoya University, 理学研究科, 准教授 (10452202)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 膜タンパク質 / 分子モーター / イオン透過 / 相互作用 |
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| Research Abstract |
Motile microorganisms, such as bacteria and archaea, sense and respond to extracellular stimuli by changing their swimming mode to migrate towards more favorite habitats. In this research, we analyzed interaction, ion transport and structural changes in a stator complex of the bacterial flagellar motor using biophysical techniques. By means of attenuated total reflection Fourier-transform infrared spectroscopy, we directly observed binding of Na^+ to carboxylates in the Pom/B complex, including the functionally essential residue Asp24 (Sudo et al.2009b, Biochemistry). We also demonstrated that Ala39-MotB and Cys31-PomB form part of the ion flux pathway (Sudo et al. 2009, Biophysics), and reported the crystal structure of C-terminal region of MotB (Kojima et al. 2009, Mol.Microbiol.). Moreover we found and characterized new receptor proteins (Suzuki et al. 2009, J.Mol.Biol., Sudo et al.2009a, Biochemistry, Yagasaki et al.2010, Biochemistry, Sudo et al. 2011, J.Biol.Chem.).
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[Journal Article] A microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties2011
Author(s)
Sudo, Y., Ihara, K., Kobayashi, S., Suzuki, D., Irieda, H., Kikukawa, T., Kandori, H., Homma, M.
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Journal Title
J.Biol.Chem 286
Pages: 5967-5976
Peer Reviewed
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[Journal Article] Spectroscopic studies of a sensory rhodopsin I homologue from the archaeon Haloarcula vallismortis2010
Author(s)
Yagasaki, J., Suzuki., D., Ihara, K., Inoue, K., Kikukawa, T., Sakai, M., Fujii, M., Homma, M., Kandori, H., Sudo, Y.
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Journal Title
Biochemistry 49
Pages: 1183-1190
Peer Reviewed
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[Journal Article] Effects of chloride ion binding on the photochemical properties of Salinibacter sensory rhodopsin I2009
Author(s)
Suzuki, D., Furutani, Y., Inoue, K., Kikukawa, T., Sakai, M., Fujii, M., Kandori, H., Homma, M., Sudo, Y.
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Journal Title
J.Mol.Biol. 392
Pages: 48-62
Peer Reviewed
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[Journal Article] Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB2009
Author(s)
Kojima, S., Imada, K., Sakuma, M., Sudo, Y., Kojima, C., Minamino, T., Homma, M., Nammba, K.
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Journal Title
Mol.Microbiol 73
Pages: 710-718
Peer Reviewed
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[Journal Article] Characterization of a signaling complex composed of sensory rhodopsin I and its cognate transducer protein from the eubacterium Salinibacter ruber2009
Author(s)
Sudo, Y., Okada, A., Suzuki, D., Inoue, K., Irieda, H., Sakai, M., Fujii, M., Furutani, Y., Kandori, H., Homma, M.
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Journal Title
Biochemistry 48
Pages: 10136-10145
Peer Reviewed
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[Journal Article] Interaction between Na(+) ion and carboxylates of the PomA-PomB stator unit studied by ATR-FTIR spectroscopy2009
Author(s)
Sudo, Y., Kitade, Y., Furutani, Y., Kojima, M., Kojima, S., Homma, M., Kandori, H.
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Journal Title
Biochemistry 48
Pages: 11699-11705
Peer Reviewed
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