2010 Fiscal Year Final Research Report
Characterization of the transient intermediate of the β2-microglobulin amyloid fibril using solution NMR techniques
| Project/Area Number |
21770169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
SAKURAI Kazumasa Osaka University, 蛋白質研究所, 助教 (10403015)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蛋白質 / 生物物理 / 自己組織化 / アミロイド線維 / NMR |
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| Research Abstract |
The purpose of this study is to clarify how β2-Microglobulin (β2m), associated with the dialysis-related amyloidosis, changes its conformation when the disease develops by using nuclear magnetic resonance. It has been believed that β2m forms amyloid fibril through a partially structured state. However, from the present data, we suggested that the partially structured state should be "activated" to be extensively unfolded state, in which the hydrophobic residues are exposed to associate with the seed.
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