2010 Fiscal Year Final Research Report
Analysis of hierarchical structure of proteins network
| Project/Area Number |
21770174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KAMIKUBO Hironari Nara Institute of Science and Technology, 物質創成科学研究科, 准教授 (20311128)
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| Project Period (FY) |
2009 – 2010
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| Keywords | X線溶液散乱 / クラスリン被覆小胞輸送 / マルチドメイン蛋白質 / ドメイン再配置 |
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| Research Abstract |
Concerning signal transduction and intracellular vesicular traffic, multi-domain proteins comprised of several functional domains act a central role in the biological functions. In this work, to elucidate the molecular mechanism of the hierarchical regulation involving multi-domain proteins, domain rearrangement of multi-domain proteins initiated by stimulating each domain, and intermolecular and intermolecular regulation associated with the domain rearrangement were examined by using x-ray scattering and dynamic light scattering. In the results, the multi-domain proteins of GGA involved in vesicular traffic and Ppr in a two component regulatory system exhibit intermolecular dissociation and association of their domains during their function expression, and the domain rearrangements are expected to regulate molecular events of protein ensembles involving the multi-domain proteins.
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[Journal Article] Cytochrome c polymerization by successive domain swapping at the C-terminal helix.2010
Author(s)
Hirota S., Hattori Y., Nagao S., Taketa M., Komori H., Kamikubo H., Wang Z., Takahashi I., Negi S., Sugiura Y., Kataoka M., Higuchi Y.
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Journal Title
Proc. Natl. Acad. Sci. USA. 107
Pages: 12854-12859
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[Presentation] Cytochrome c polymerization by successive domain swapping at the C-terminal helix.2010
Author(s)
Z.Wang, Y.Hattori, S.Nagao, M.Takeda, H.Komori, H.Kamikubo, I.Takahashi, S.Negi, Y.Sugiura, M.Kataoka, Y.Higuchi, S.Hirota.
Organizer
The 4th International Symposium "Molecuar Science of Fluctuations toward Biological Functions"
Place of Presentation
Otsu, Japan
Year and Date
20101130-20101201
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