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2010 Fiscal Year Final Research Report

Molecular mechanisms of transcriptional regulation via histone acetylation

Research Project

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Project/Area Number 21770181
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

FUKUDA Aya  University of Tsukuba, 大学院・人間総合科学研究科, 助教 (50436276)

Project Period (FY) 2009 – 2010
Keywords転写 / クロマチン / ヒストン / アセチル化
Research Abstract

To understand molecular mechanisms of transcriptional regulation via histone modifications, I have tried to identify the molecule(s) that is required for transcription from the chromatin template and have been focusing histone acetylation to analyze the relationship between histone modifications and transcriptional regulation. Using a purified in vitro transcription system, multiple chromatin transcription-enabling activities were identified in HeLa nuclear extract-derived fractions. Also, some of the acetylation-deficient mutants of histone H3 or H4 showed different transcription activity from the wild-type histone, suggesting that acetylation of the specific sites is important for transcriptional regulation.

  • Research Products

    (12 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Book (1 results) Remarks (1 results)

  • [Journal Article] cAMP-response element-binding protein (CREB) controls MSK1-mediated phosphorylation of histone H3 at the c-fos promoter in vitro2010

    • Author(s)
      Shimada M, Nakadai T, Fukuda A, Hisatake K.
    • Journal Title

      J.Biol.Chem. 285(13)

      Pages: 9390-9401

    • Peer Reviewed
  • [Journal Article] Heterogeneous nuclear ribonucleoprotein R enhances transcription from the naturally configured c-fos promoter in vitro2009

    • Author(s)
      Fukuda A, Nakadai T, Shimada M, Hisatake K.
    • Journal Title

      J.Biol.Chem. 284(35)

      Pages: 23472-23480

    • Peer Reviewed
  • [Presentation] Mechanism of c-fos transcriptional regulation by an RNA-binding protein hnRNP R2010

    • Author(s)
      Hisatake K, Takeuchi M, Nakadai T, Shimada M, Fukuda A.
    • Organizer
      ASBMB Special Symposia "Transcriptional Regulation by Chromatin and RNA Polymerase II, Granlibakken Resort
    • Place of Presentation
      Tahoe City, USA
    • Year and Date
      20100930-20101004
  • [Presentation] Mechanistic analysis of c-fos transcriptional regulation by a novel coactivator hnRNP R2010

    • Author(s)
      Fukuda A, Takeuchi M, Nakadai T, Shimada M, Hisatake K.
    • Organizer
      9th EMBL Conference Transcription and Chromatin, EMBL Heidelberg
    • Place of Presentation
      Germany
    • Year and Date
      20100828-20100831
  • [Presentation] Mechanistic analysis of c-fos transcriptional regulation by a novel coactivator hnRNP R2010

    • Author(s)
      Fukuda A, Takeuchi M, Nakadai T, Shimada M, Hisatake K
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
  • [Presentation] Mechanistic analyses of transcriptional regulation of the c-fos gene via the NF complexes2010

    • Author(s)
      Nakadai T, Fukuda A, Takeuchi M, Shimada M, Hisatake K
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] Mechanism of c-fos transcriptional regulation by an RNA-binding protein, hnRNP R2010

    • Author(s)
      Hisatake K, Takeuchi M, Nakadai T, Shimada M, Fukuda A
    • Organizer
      Leading Graduate Schools International Conference
    • Place of Presentation
      つくば
    • Year and Date
      2010-11-01
  • [Presentation] Transcriptional regulation of c-fos gene via RNA binding proteins2009

    • Author(s)
      Fukuda A, Nakadai T, Shimada M, Hisatake K.
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] RNA binding proteins regulate the c-fos transcription2009

    • Author(s)
      Fukuda A, Nakadai T, Shimada M, Hisatake K.
    • Organizer
      Cold Spring Harbor Laboratory meeting on "Mechanisms of Eukaryotic Transcription"
    • Place of Presentation
      New York
    • Year and Date
      20090825-20090829
  • [Presentation] Transcriptional Regulation by RNA Binding Proteins2009

    • Author(s)
      Fukuda A, Nakadai T, Shimada M, Hisatake K.
    • Organizer
      日本生化学会関東支部例会
    • Place of Presentation
      つくば
    • Year and Date
      2009-06-20
  • [Book] 実験医学別冊(「目的別で選べるタンパク質発現プロトコール」(「昆虫細胞」の項))2010

    • Author(s)
      福田綾、久武幸司
    • Total Pages
      58-65(120-144)
    • Publisher
      羊土社
  • [Remarks] ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/biochem/gene/index.html

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Published: 2012-02-13   Modified: 2016-04-21  

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