2010 Fiscal Year Final Research Report
Molecular mechanisms of transcriptional regulation via histone acetylation
| Project/Area Number |
21770181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
FUKUDA Aya University of Tsukuba, 大学院・人間総合科学研究科, 助教 (50436276)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 転写 / クロマチン / ヒストン / アセチル化 |
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| Research Abstract |
To understand molecular mechanisms of transcriptional regulation via histone modifications, I have tried to identify the molecule(s) that is required for transcription from the chromatin template and have been focusing histone acetylation to analyze the relationship between histone modifications and transcriptional regulation. Using a purified in vitro transcription system, multiple chromatin transcription-enabling activities were identified in HeLa nuclear extract-derived fractions. Also, some of the acetylation-deficient mutants of histone H3 or H4 showed different transcription activity from the wild-type histone, suggesting that acetylation of the specific sites is important for transcriptional regulation.
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