2011 Fiscal Year Final Research Report
Molecular mechanism of transcriptional regulation of ribosomal protein genes in budding yeast
| Project/Area Number |
21770189
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
KASAHARA Koji 横浜市立大学, 生命ナノシステム科学研究科, 客員研究員 (40304159)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | リボソーム / 転写 / RNAポリメラーゼTT / 出芽酵母 / ヌクレオソーム / PTC(転写開始前複合体) / Hmo1 |
|---|
| Research Abstract |
Hmo1, a HMGB family protein of Saccharomyces cerevisiae, specifically binds to the promoters of ribosomal protein genes, and is involved in the transcriptional activation and determination of transcription start site for these promoters. Our analyses for the function and a binding site of Hmo1 on the RPS5 promoters revealed that Hmo1 and +1 nucleosome determine the 5'- and 3'-boudaries, respectively, , of a zone available for PIC assembly, thereby directing PIC assembly at a billogically relevant sote.
|
