2010 Fiscal Year Final Research Report
Analysis of protein interaction networks by photo-cross-linking
| Project/Area Number |
21770195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SATO Aya The Institute of Physical and Chemical Research, 拡張遺伝暗号システム研究チーム, 特別研究員 (30469902)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蛋白質 / 光クロスリンク / 非天然型アミノ酸 / 分子間相互作用 |
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| Research Abstract |
"The photo-cross-linking method" is the new technique that can stabilized protein complexes formed in living cells to isolate and analyze them. In this study, I identified the efficient positions to incorporate photo-cross-linkers in several protein-binding domains for the purpose of applying the photo-cross-linking method to the elucidation of the protein interaction networks.
Furthermore, I succeeded in the crystallographic study of the cross-linked protein complex.
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