2011 Fiscal Year Final Research Report
Functional analysis of actin-binding protein Tropomyosin4 in the spindle formation and orientation
| Project/Area Number |
21770200
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 減数分裂 / 紡錘体 / リン酸化 / アクチン / マウス卵 |
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| Research Abstract |
The mammalian oocyte is highly polarized because asymmetrical spindle migration to the oocyte cortex ensures extrusion of small polar bodies in the two meiotic divisions. To elucidate the mechanism of maintain this polarity, I analyzed the function of an actin-filament associated protein tropomyosin 4(Tm4). Tm4-depletion by siRNA causes abnormal spindles with diffused poles and polarity of spindles in oocyte. These data indicate that Tm4 plays important roles in spindle orientation and chromosome migration during meiosis.
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