2010 Fiscal Year Final Research Report

A novel activity of HIV-1 Rev protein to remodel export RNPs in directing virus-specific RNA export

Research Project

Project/Area Number	21770204
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Cell biology
Research Institution	Kyoto University
Principal Investigator	TANIGUCHI Ichiro Kyoto University, ウイルス研究所, 助教 (00467432)
Project Period (FY)	2009 – 2010
Keywords	RNA核外輸送 / mRNAスプライシング / RNA-タンパク質複合体
Research Abstract	HIV-1 Rev protein induces export of singly spliced HIV-1 transcript (4kb-RNA) that is normally retained in the nucleus. However, how 4kb-RNA is exported is not well understood, since such a transcript should have possibility of utilizing CRM1-dependent or TAP-dependent export, or both. To understand the export mechanism, we generated model RNA substrates that recapitulated the situation of export of 4kb-RNA in Xenopus oocytes, and investigated the action of Rev in detail. We found that such model RNA substrates utilized exclusively CRM1-dependent export pathway in the presence of Rev. Most importantly, this commitment to a single RNA export pathway is achieved by Rev's ability not only to induce CRM1-dependent export but also to suppress TAP-dependent export.

(3 results)