2010 Fiscal Year Final Research Report
The activation mechanism of a membrane-bound transcription factor ATF6 in response to Endoplasmic Reticulum Stress
| Project/Area Number |
21770205
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Kyoto University |
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Principal Investigator |
OKADA Tetsuya Kyoto University, 大学院・理学研究科, 助教 (70378529)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 小胞体ストレス / ATF6 / DT40 |
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| Research Abstract |
A membrane-bound transcription factor ATF6 is activated by accumulation of unfolded proteins in the endoplasmic reticulum (ER). We previously found that p45, a membrane protein localized in the ER, binds to ATF6 in response to the accumulation of unfolded proteins. In this study, we obtained following results.
(1) We found that the p45-homologous protein, p50, also binds to ATF6 in the ER stress dependent manner, and that p45 and p50 bind to ATF6 through their N-terminal regions. (2) We established p50-deficient cells using the chicken DT40 cell line. (3) We established a system to identify components of ATF6 complex by mass spectrometry.
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