The analysis of membrane interface in the formation of the cell-cell adhesion complex

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YAMAZAKI Yuji Osaka University, 大学院・生命機能研究科, 助教 (80527664)
• Project Period (FY): 2009 – 2010
• Keywords: タイトジャンクション / シグナル / ZO-1 / アドヘレンスジャンクション / アクトミオシンリング

Research Abstract

The epithelial cell-cell adhesion complex, which consists of belt-like adherens junctions (AJ) and tight junctions (TJ), is thought to be an important factor in many molecular events. The role of this complex comes from spatio-temporal regulation that leads to AJ and TJ formation, a process that is poorly understood. In this project, we analyzed the FRAP dynamics of claudins and ZO-1 to reveal their spatiotemporal behaviors and interactions in epithelial cells, and use these observations as the basis for a new scheme to describe their molecular interactions. We examined the spatiotemporal regulation of AJ/TJ formation and modulation, finding new spatiotemporal signaling for AJ regulation that includes the identification of Tara, a GEF binding protein, as an AJ protein.

Research Products

• [Journal Article] Role of claudin species-specific dynamics in reconstitution and remodeling of the zonula occludens. (2011)
  • Author(s): Yamazaki, Y.#, Tokumasu, R#., Kimura, H., Tsukita, S.
  • Journal Title: Mol.Biol.Cell 22 Pages: 1495-1504
  • Peer Reviewed
• [Journal Article] Tara up-regulates E-cadherin transcription by binding to theTrio RhoGEF and inhibiting Rac signaling. (2011)
  • Author(s): Yano, T.#, Yamazaki, Y.#, Adachi, M., Okawa, K., Fort, P., Uji, M., Tsukita, S., Tsukita, S.^*
  • Journal Title: J.Cell Biol. 193 Pages: 319-332
  • Peer Reviewed
• [Journal Article] Predicted expansion of the claudin multigene family. (2011)
  • Author(s): Mineta, K.^*, Yamamoto, Y., Yamazaki, Y.,Tanaka, H., Tada, Y., Saito, K., Tamura,A, Igarashi, M., Endo, T., Takeuchi, K., Tsukita, S.^*
  • Journal Title: FEBS letters 585 Pages: 606-612
  • Peer Reviewed
• [Journal Article] Loss of claudin-15, but not claudin-2, causes Na+ deficiency and Glucose malabsorption in mouse small intestine. (2011)
  • Author(s): Tamura, A., Hayashi, H., Imasato, M., Yamazaki, Y., Hagiwara, A., Wada, M., Noda,T., Watanabe, M., Suzuki, Y., Tsukita, S.^*
  • Journal Title: Gastroenterology 140 Pages: 913-923
  • Peer Reviewed
• [Journal Article] Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function. (2009)
  • Author(s): Tsukita, S., Katsuno, T., Yamazaki, Y., Umeda, K., Tamura, A., Tsukita, S.
  • Journal Title: Ann.N.Y.Acad.Sci. 1165 Pages: 44-52
• [Presentation] Novel TBP/Trio/Rac1/p38/Tbx3 cascade regulates E-cadherin expression to fine-tune the epithelial cell sheet (2010)
  • Author(s): Yuji Yamazaki
  • Organizer: Membrane dynamics of the cell.
  • Place of Presentation: Heinrich Heine University, Germany
  • Year and Date: 2010-09-23
• [Presentation] クローディンによるストランド形成のダイナミクス (2010)
  • Author(s): 山崎裕自
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-21
• [Presentation] Roles of ZO1/2 in the Formation of Epithelial Cell-cell Junctions and Paracellular Transport (2009)
  • Author(s): Yuji Yamazaki
  • Organizer: IUPS2009
  • Place of Presentation: 京都国際会館
  • Year and Date: 2009-07-30
• [Book] Water-based biological homeostasis in multiple fluid-compartments in the higher organisms : regulation by the paracellular barrier-forming tight junction of epithelial cell sheets. (2010)
  • Author(s): Yamazaki, Y., Tamura, A., Tsukita, S.
  • Total Pages: 247-258
  • Publisher: In Water : The Forgotten Biological Molecule PanStanford Publish
• [Remarks] 月田研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/