2010 Fiscal Year Final Research Report
Targeting mechanism of Drpl to mitochondrial fission sites
| Project/Area Number |
21770214
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Kyushu University |
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Principal Investigator |
OTERA Hidenori Kyushu University, 医学研究院, 助教 (40380612)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 生体膜 |
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| Research Abstract |
Cytoplasmic dynamin-related GTPase Drp1 is recruited to mitochondria and mediates mitochondrial fission. Although mitochondrial outer membrane (MOM) protein Fis 1 is thought to be a Dip 1 receptor, this has not been confirmed. To analyze the mechanism of Drp 1 recruitment, we manipulated expression of mitochondria] fission and fusion proteins and demonstrated that (i) mitochondrial fission factor (Mff)-knockdown released the Drp 1 foci from MOM accompanied by network extension, while Mff-overexpression stimulated mitochondrial recruitment of Drpl accompanied by mitochondrial fission ; (ii) Mff-dependent mitochondrial fission proceeded independent of Fisl ; (iii) Mff mutant with plasma membrane-targeted CAAX motif directed Drpl to the target membrane ; (iv) Mff and Drpl physically interacted in vitro and in vivo ; (v) exogenous stimuli-induced mitochondrial fission and apoptosis were compromised by knockdown of Drp1 and Mff, but not Fisl ; and (vi) conditional knockout of Fis l in colon carcinoma cells revealed that it is dispensable for mitochondrial fission. Thus, Mff, but not hFis 1, functions as an essential factor in mitochondrial recruitment of Drp 1.
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Research Products
(10 results)
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[Journal Article] Mitochondrial fission factor Drpl is essential for embryonic development and synapse formation in mice.2009
Author(s)
Ishihara N, Nomura M, Jofuku A, Kato H, Suzuki SO, Masuda K, Otera H, Nakanishi Y, Nonaka I, Goto YI, Taguchi N, Morinaga H, Maeda M, Takayanagi R, Yokota S, Mihara K.
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Journal Title
Nat Cell Biol. Aug 11(8)
Pages: 958-966
Peer Reviewed
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