2010 Fiscal Year Final Research Report
Elucidation of mechanisms for cell migration by visualization of PLD activity and monitoring of single molecule dynamics in living cells.
| Project/Area Number |
21770223
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NAGASAKI Akira National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究員 (30392640)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 細胞骨格 / 運動 |
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| Research Abstract |
Phospholipase D (PLD) is an enzyme that catalyzes the hydrolysis of a major component of membrane phospholipids, phosphatidylcholine, to generate a second messenger phosphatidic acid (PA). We have already shown that PLD gene is required for cell migration in NBT-II cells and Dictyostelium cells. To elucidate the role of PLD in migrating cells, we tried to construct probes for detection of PLD activity and to observe the single molecular dynamics of PLD on cell membrane.
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