2010 Fiscal Year Final Research Report

Functional analysis of nucleostemin in pluripotent stem and tissue stem cells

Research Project

Project/Area Number	21770242
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Developmental biology
Research Institution	Keio University
Principal Investigator	NAGAMATSU Go Keio University, 医学部, 助教 (70453545)
Project Period (FY)	2009 – 2010
Keywords	幹細胞 / 自己複製
Research Abstract	We succeeded in making the conditional Nucleostemin deficient embryonic stem cells by a consecutive targeting of two times. Upon deletion of Nucleostemin allele, stop of proliferation and cell death had found. Moreover, it was clarified that the genes that did an important working to the maintenance of the undifferentiation of the embryonic stem cell, such as Oct3/4, Nanog, and Sox2 decreased along with the Nucleostemin loss. The possibility of the inducement or maintaining of the factors that Nucleostemin is related to the above-mentioned undifferentiated maintenance is suggested from this. On the other hand when Nucleostemin was over-expressed, the up-regulation of pluripotency associated genes was observed.