Analysis of non-muscle myosin II in left-right asymmetric morphogenesis of internal organ

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770243
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Gakushuin University (2010); Tokyo University of Science (2009)
• Principal Investigator: OKUMURA Takashi 学習院大学, 理学部, PD 共同研究員 (20449234)
• Project Period (FY): 2009 – 2010
• Keywords: 左右非対称性 / ショウジョウバエ / 非筋定型ミオシンII / 消化管 / 形態形成 / Zipper

Research Abstract

Many animals exhibit stereotypical left-right (LR) asymmetry in their internal organs. From our genetic analysis, activation of non-muscle myosin II has been suggested to be required for LR asymmetric morphogenesis of the Drosophila embryonic midgut. In this study, we try to ask whether activation of non-muscle myosin II show LR bias in the LR morphogenesis. As a result, we could not clearly confirm its LR-biased activation, but we demonstrated that LR asymmetric cell movement of the midgut depended on non-muscle myosin II activation.

Research Products

• [Journal Article] A novel Cre/loxP system for mosaic gene expression in the Drosophila embryo. (2012)
  • Author(s): Naotaka Nakazawa, Kiichiro Taniguchi, Takashi Okumura, Reo Maeda, Kenji Matsuno.
  • Journal Title: Developmental Dynamics. Volume: 241(5) Pages: 965-974
• [Journal Article] CanonicalWnt signaling in the visceral muscle is required for left-right asymmetric development of the Drosophila midgut. (2011)
  • Author(s): Junpei Kuroda, Mitsutoshi Nakamura, Masashi Yoshida, Haruka Yamamoto, Takayoshi Maeda, Kichiro Taniguchi, Naotaka Nakazawa, Ryo Hatori, Akira Ishio, Ayumi Ozaki, Shunsuke Shimaoka, Tamiko Ito, Hironao Iida, Takashi Okumura, Reo Maeda, Kenji Matsuno.
  • Journal Title: Mechanism of Development. Volume: 128 (11-12) Pages: 625-639
• [Journal Article] Chirality in planar cell shape contributes to left-right asymmetric epithelial morphogenesis. (2011)
  • Author(s): Kiichiro Taniguchi, Reo Maeda, Tadashi Ando, Takashi Okumura, Naotaka Nakazawa, Ryo Hatori, Mitsutoshi Nakamura, Shunya Hozumi, Hiroo Fujiwara, Kenji Matsuno
  • Journal Title: Science Volume: 333 Pages: 339-341
• [Journal Article] Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. (2010)
  • Author(s): Takashi Okumura, Hiroo Fujiwara, Kichiro Taniguchi, Junpei Kuroda, Naotaka Nakazawa, Mitsutoshi Nakamura, Ryo Hatori, Akira Ishio, Reo Maeda, Kenji Matsuno.
  • Journal Title: Developmental bioloby Volume: 344 Pages: 693-706
• [Presentation] A Drosophila GATA transcription factor gene, GATAe, is required for maintaining adult intestinal stem cells (2011)
  • Author(s): Takashi Okumura and Takashi Adachi-Yamada
  • Organizer: 1st Asia-Pasific Drosophila Research Conference (APDRC)
  • Place of Presentation: 台北(台湾)
  • Year and Date: 20110522-25
• [Presentation] A Drosophila GATA transcription factor gene, GATAe, is required for maintaining adult intestinal stem cells (2011)
  • Author(s): Takashi Okumura and Takashi Adachi-Yamada
  • Organizer: 日本発生生物学会第 44 回大会
  • Place of Presentation: 沖縄県, 沖縄コンベンションセンター
  • Year and Date: 20110518-21
• [Presentation] Functional analysis of three class I myosin genes in Drosophila development (2009)
  • Author(s): Takashi Okumura, Shunya Hozumi, Kiichiro Taniguchi, Reo Maeda, Kenji Matsuno
  • Organizer: 第9 回日本ショウジョウバエ研究会
  • Place of Presentation: 静岡県 ヤマハリゾート つま恋
  • Year and Date: 20090706-08
• [Presentation] Three class I myosin genes have redundant and distinct function in Drosophila development (2009)
  • Author(s): Takashi Okumura, Shunya Hozumi, Kiichiro Taniguchi, Reo Maeda, Kenji Matsuno
  • Organizer: 日本発生生物学会 第42 回大会
  • Place of Presentation: 新潟県 朱鷺メッセ
  • Year and Date: 20090528-31