2011 Fiscal Year Final Research Report
Development of an efficient chromatin pull-down method from small cell numbers
| Project/Area Number |
21770251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University (2010-2011)
The Institute of Physical and Chemical Research (2009) |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生殖細胞 / エピジェネティクス |
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| Research Abstract |
The aim of this research project is to develop a method to determine the binding sites of specific transcription factors that play important roles in specific cell lineages, such as primordial germ cells. To purify genomic DNA bound to a specific transcription factor, I developed knock-in mice that carry tags in the particular transcription factor. Furthermore, I developed a method to quantitatively amplify genomic DNA bound to a transcription factor. By these methods, it has become plausible to determine genomic sites bound to a transcription factor in a small cell number, such as 10, 000 cells.
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[Journal Article] TDRD5is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice2011
Author(s)
Yabuta, Y., Ohta, H., Abe, T., Kurimoto, K., Chuma, S., and Saitou, M.
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Journal Title
J Cell Biol
Volume: 192
Pages: 781-795
DOI
Peer Reviewed
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[Journal Article] Conditional knockdown of Nanoginduces apoptotic cell death in mouse migrating primordial germ cells2009
Author(s)
Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M., and Tada, T.
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Journal Title
Development
Volume: 136
Pages: 4011-4020
DOI
Peer Reviewed
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