Analysis of bacteriophage Qβ genome evolution in an experimental coevolution system

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Hirosaki University
• Principal Investigator: KASHIWAGI Akiko 弘前大学, 農学生命科学部, 准教授 (40362652)
• Project Period (FY): 2009 – 2010
• Keywords: 共進化 / 実験進化 / Qβファージ / 大腸菌

Research Abstract

How does evolution affect changes in parasite genome sequences? To elucidate the effects of coevolution, we constructed an experimental coevolution system and sole parasite evolution system consisting of Escherichia coli and the lytic RNA bacteriophage Qβ (Qβ phage). We passaged Qβ phage in both systems for approximately 170 phage generations. Comparison of sequential (time-dependent) changes in the Qβ phage genome sequence in both evolution systems revealed that the coevolution system showed about 3.4-fold higher fixation rate.

Research Products

• [Journal Article] PLoS ONE (2011)
  • Author(s): Kazufumi Hosoda, Shingo Suzuki, Yoshinori Yamauchi, Yasunori Shiroguchi, Akiko Kashiwagi, Naoaki Ono, Kotaro Mori, Tetsuya Yomo
  • Journal Title: issue 2 Volume: volume 6 Pages: e17105
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Kumiko Kihara, Kotaro Mori, Shingo Suzuki, Kazufumi Hosoda, Akito Yamada, Shin'ichi Matsuyama, Akiko Kashiwagi, Tetsuya Yomo
  • Journal Title: Bio Systems Volume: vol.13 Pages: 342-347
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Kotaro Mori, Akiko Kashiwagi, Tetsuya Yomo
  • Journal Title: The Journal of Eukaryotic Microbiology Volume: vol.58(1) Pages: 37-42
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Toshihiko Kishimoto, Leo Iijima, Makoto Tatsumi, Naoaki Ono, Ayana Oyake, Tomomi Hashimoto, Moe Matsuo, Masato Okubo, Shingo Suzuki, Kotaro Mori, Akiko Kashiwagi, Chikara Furusawa, Bei-Wen Ying, Tetsuya Yomo
  • Journal Title: PLoS Genetics Volume: vol.6(10) Pages: e1001164
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Saburo Tsuru, Junya Ichinose, Akiko Kashiwagi, Bei-Wen Ying, Kunihiko Kaneko, Tetsuya Yomo
  • Journal Title: Physical Biology Volume: vol.6 Pages: 036015(9pp)
  • Peer Reviewed
• [Presentation] ワールドコンベンションセンターサミット (2010)
  • Author(s): 柏木明子、四方哲也
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: フェニックス・シーガイア・リゾート(宮崎県山崎町浜山)
  • Year and Date: 20101027-29
• [Presentation] 日本遺伝学会第82回大会 (2010)
  • Author(s): 柏木明子、四方哲也
  • Organizer: 日本遺伝学会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 20100920-22
• [Presentation] 第12回日本進化学会大会 (2010)
  • Author(s): 柏木明子、四方哲也
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 20100802-0805
• [Presentation] APBioChEC’09 (2009)
  • Author(s): Akiko Kashiwagi, Takahiro Sakurai, Saburo Tsuru, Bei-Wen Ying, Kotaro Mori, Tetsuya Yomo
  • Place of Presentation: Kobe, JAPAN
  • Year and Date: 20091124-28
• [Presentation] International Symposium on COMPLEX SYSTEMS BIOLOGY (2009)
  • Author(s): Akiko Kashiwagi
  • Place of Presentation: The University of Tokyo, Hongo Campus
  • Year and Date: 20090929-1001
• [Presentation] 第11回日本進化学会大会(札幌大会) (2009)
  • Author(s): 柏木明子、四方哲也
  • Place of Presentation: 北海道大学高等教育機能開発総合センター
  • Year and Date: 20090902-04
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.ac.jp/kohou2/public/oyo/a.kashiwagi/index.htm