2011 Fiscal Year Final Research Report
Molecular breeding of tryptophan-fortified rice via gene targeting
| Project/Area Number |
21780010
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | National Institute of Agrobiological Sciences |
|---|
Principal Investigator |
SAIKA Hiroaki 独立行政法人農業生物資源研究所, ゲノム機能改変研究ユニット, 主任研究員 (20435613)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 遺伝子ターゲッティング / 点変異 / 代謝改変 / トリプトファン / 相同組換え / 遺伝子組換え植物 / アミノ酸アナログ |
|---|
| Research Abstract |
Gene targeting(GT) via homologous recombination is a powerful transformation technology because it enables to modify the endogenous target gene as expected. In this study, we succeeded in the production of novel tryptophan(Trp)-fortified rice plants by GT-mediated targeted mutagenesis of OASA2 gene that encodes a key enzyme in Trp biosynthesis of rice. In the GT plant, other amino acids except for Trp and the metabolites synthesized from Trp such as serotonin, vitamin B and indole alkaloid accumulated at a higher level also. Thus, we demonstrated that GT-mediated targeted mutagenesis is applicable to metabolic engineering in crops.
|
