2011 Fiscal Year Final Research Report

Study of anthocyanin modification genes that cause flower color diversity and development of techniques for the gene regulation

Research Project

Project/Area Number	21780033
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Horticulture/Landscape architecture
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	NODA Naonobu 独立行政法人農業・食品産業技術総合研究機構, 花き研究所花き研究領域, 主任研究員 (10455313)
Project Period (FY)	2009 – 2011
Keywords	花色 / 遺伝子組換え / アントシアニン / 糖転移酵素 / アシル基転移酵素
Research Abstract	The glycosyltransferase(GT) and acyltransferase(AT) genes involved in anthocyanin biosynthesis were cloned and transformed into chrysanthemums to development of techniques for the gene regulation. We cloned genes that probably encode acyl-CoA : anthocyanin 3-or 5-acyltransferase from Lobelia. Perilla anthocyanin 3AT was suitable for aromatic acylation to the glucose of anthocyanin position 3 in chrysanthemum flowers. The petals of transgenic chrysanthemums that introduced petal specific promoter driven and translational enhancer fused Clitoria 3' 5' GT were synthesized and accumulated B-ring glycosylated anthocyanins. We also generated transgenic chrysanthemum plants co-expressing genes for B-ring hydroxylation, glycosylation and acylation. In the near future, we will characterize these transgenic chrysanthemum lines.