2010 Fiscal Year Final Research Report
Investigation of host genes involved in cross-protection between plant viruses
| Project/Area Number |
21780040
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKESHITA Minoru Kyushu University, 大学院・農学研究院, 助教 (00304767)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 植物ウィルス / 干渉効果 / 宿主 / 遺伝子 / 競合 |
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| Research Abstract |
To dissect the molecular mechanisms of plant viral interference, host genes were screened using the T-DNA tagged lines of Arabidopsis thaliana. Cucumber mosaic virus (CMV) vectors expressing fluorescent proteins were used for inoculation onto the A.thaliana lines All the A. thaliana lines examined in this study showed neither break down nor compromise of virus interference.
We further examined the roles of the RDR1 and the IVR genes in virus resistances. The RDR1 gene showed inhibitory acts multiplication of Potato virus Y.However the RDR1 gene did not affect plant viral interference. Low temperature and plant viral synergism also did not compromise the interference. The results suggested that RNA silencing machinery does not contribute greatly to the phenomenon.
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