2011 Fiscal Year Final Research Report
Novel pyrethroid-metabolizing enzyme in the silkmoth
| Project/Area Number |
21780049
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | Kyushu University |
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Principal Investigator |
KOHJI Yamamoto 九州大学, 大学院・農学研究院, 助教 (00346834)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 環境 / 解毒酵素 / 昆虫 / マイクロアレイ / 農薬 |
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| Research Abstract |
Glutathione transferases comprise a major family of detoxification enzyme. Here, we report the substrate specificity, the crystal structure of unclassified GST of Bombyx mori, bmGSTU, and showing that it occurred as a dimer, like other GSTs. The bmGSTU was able to conjugate glutathione to 1-chloro-2, 4-dinitrobenzene, the universal substrate of GST. The structure of bmGSTU was determined with resolutions of 2. 1 A, by synchrotron radiation and molecular replacement method. Each subunit of bmGSTU displayed a glutathione-binding site in the active center. Furthermore, the bmGSTU mRNA was highly expressed in insecticide-resistance strain of B. mori.
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Research Products
(18 results)
