2011 Fiscal Year Final Research Report
Localization of Candidatus Liberibacter asiaticus in Diaphorina citri(Hemiptera : Psyllidae)
| Project/Area Number |
21780051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
INOUE Hiromitsu 独立行政法人農業・食品産業技術総合研究機構, 果樹研究所・カンキツ研究領域, 主任研究員 (80414663)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 昆虫分子生物学 / 虫媒伝染性植物病害 / in situハイブリダイゼーション |
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| Research Abstract |
Diaphorina citri will not always transmit Candidatus Liberibacter asiaticus(Las), the bacterium associated with the disease of huanglongbing or citrus greening, if the vector fed on infected citrus tree. We investigated the localization of Las in Las-positive but non-transmissible D. citri adults by oligonucleotide-probed in situ hybridization targeted to the bacterial 16S ribosomal RNA. The significant signals showing the targets were detected in the filter chamber, midgut, ovalies in tissue sections of non-transmissible Las-positive D. citri.
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[Journal Article] Enhanced proliferation and efficient transmission of Candidatus Liberibacter asiaticus by adult Diaphorina citri after acquisition feeding in the nymphal stage2009
Author(s)
Inoue, H., Ohnishi, J., Ito, T., Tomimura, K., Miyata, S., Iwanami, T., Ashihara, W.
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Journal Title
Annals of Applied Biology
Volume: 155
Pages: 29-36
DOI
Peer Reviewed
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