2011 Fiscal Year Final Research Report
Genetic engineering for production of copolymerized biopolyester from C1compounds
| Project/Area Number |
21780066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
ORITA Izumi 東京工業大学, 大学院・生命理工学研究科, 助教 (70525964)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ポリヒドロキシアルカン酸 / C_1化合物 / メタノール資化性細菌 |
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| Research Abstract |
This study is aimed at construction of recombinant methylotrophic bacteria to produce flexible polyhydroxyalkanoate(PHA) copolymers from methanol using Methylobacterium extorquens AM1 as a host strain. The results revealed that M. extorquens wild-type strain accumulates PHA copolymer composed of mostly C4 monomer with a small amount of C_5 unit. The recombinant strains, which can accumulate PHA composed of C_4, C_5, and C_6 unit, was successfully obtained. These strains produced PHA more efficiently than wild-type strain.
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