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2010 Fiscal Year Final Research Report

A novel reduction-stress elimination system by the Escherichia coli DsbA and its application to L-cysteine production

Research Project

  • PDF
Project/Area Number 21780073
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

OHTSU Iwao  Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (60395655)

Co-Investigator(Renkei-kenkyūsha) MORI Hirotada  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90182203)
Project Period (FY) 2009 – 2010
Keywords発酵生産
Research Abstract

DsbA oxidizes peptidyl L-cysteine residue into disulfide bridge in the periplasm, and the reduced form of DsbA is re-oxidized by inner membrane protein DsbB. Electron, which is received from ubiquinone, is given to DsbA. Finally, oxygen is an electron acceptor through the terminal oxidase of the respiratory chain (DsbA-DsbB-UQ complex). We find that DsbA, which is normally the oxidized form, is shifted in the reduced form of DsbA in the addition of excess Cys. Furthermore, OstA protein, which is one of the DsbA substrates and is an essential protein for Escherichia coli growth, is also accumulated as reduced form into the periplasm. We suggested that the DsbA-DsbB-ubiquinone oxidation system might eliminate reductive stress by oxidizing exogenous reductants and also play an important role in redox homeostasis in the cytoplasm. A similar mechanism to DsbA-DsbB-ubiquinone oxidation system of E. coli exists widely also in endoplasmic reticulum of yeast, the plant, and the animal, etc. and mitochondria. This is corresponding to the toxicity of Cys that exceeds the living thing kind.

  • Research Products

    (12 results)

All 2011 2010 2009 Other

All Journal Article (3 results) Presentation (7 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] システイン/シスチンのシャトルシステムによる新しい酸化ストレス防御機構2011

    • Author(s)
      大津厳生, 高木博史
    • Journal Title

      化学と生物 49

      Pages: 81-83

  • [Journal Article] The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Esoherichia coli.2010

    • Author(s)
      Iwao Ohtsu^*, Natthawut Wiriyathanawudhiwong, Susumu Morigasaki, Takeshi Nakatani, Hiroshi Kadokura, Hiroshi Takagi
    • Journal Title

      J.Biol.Chem. 285

      Pages: 17479-17487

  • [Journal Article] The outer membrane TolC is involved in cysteine tolerance and overproduction in Esoherichia coli.2009

    • Author(s)
      Natthawut Wiriyathanawudhiwong^*, Iwao Ohtsu^*, Zhao-Di Li, Hirotada Mori, Hiroshi Takagi
    • Journal Title

      Appl.Microbiol.Bioteoh. 81

      Pages: 903-913

  • [Presentation] システインによる大腸菌の生育阻害機構の解析2011

    • Author(s)
      佐々木翠、大津厳生、高木博史
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-03-28
  • [Presentation] システインによる大腸菌の生育阻害機構の解析2010

    • Author(s)
      佐々木翠, Natthawut Wiriyathanawudhiwong, 大津厳生, 高木博史
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-09
  • [Presentation] システイン/シスチンのシャトルシステムによるユニークな酸化ストレス防御機構2010

    • Author(s)
      鈴木茉里奈, Natthawut Wiriyathanawudhiwong, 大津厳生, 高木博史
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-09
  • [Presentation] システインによる大腸菌の生育阻害機構の解析2010

    • Author(s)
      佐々木翠, Natthawut Wiriyathanawudhiwong, 大津厳生, 高木博史
    • Organizer
      日本農芸化学会関西支部例会(第467回講演会)
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-04
  • [Presentation] 大腸菌のペリプラズムにおける過酸化水素の消去機構2010

    • Author(s)
      大津厳生、Natthawut Wiriyathanawudhiwong、鈴木茉里奈、高木博史
    • Organizer
      第4回ゲノム微生物学会若手の会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-10-01
  • [Presentation] 大腸菌ペリプラズム内におけるシステインの生理的役割2010

    • Author(s)
      大津厳生, Natthawut Wiriyathanawudhiwong, 高木博史
    • Organizer
      第10回日本蛋白質科学会年会,「レドックスバイオロジーの最前線~蛋白質科学と細胞生物学の接点~」
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-16
  • [Presentation] 大腸菌ペリプラズム内におけるシステインの生理的役割2010

    • Author(s)
      大津厳生, Natthawut Wiriyathanawudhiwong, 高木博史
    • Organizer
      第57回日本生化学会近畿支部例会
    • Place of Presentation
      生駒市
    • Year and Date
      2010-05-22
  • [Remarks] ホームページ等

    • URL

      http://bsw3.naist.jp/takagi/takagi-j.html

  • [Patent(Industrial Property Rights)] L-システイン生産菌及びL-システインの製造法2009

    • Inventor(s)
      大津厳生, 高木博史, 野中源
    • Industrial Property Rights Holder
      味の素株式会社
    • Industrial Property Number
      国際公開WO 2009/104731 A1
    • Filing Date
      2009-08-27

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Published: 2012-02-13   Modified: 2016-04-21  

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