2010 Fiscal Year Final Research Report
The regulation of metabolic pathways by polyphosphate accumulation and its application to fermentation technology
| Project/Area Number |
21780075
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
HIROTA Ryuichi Hiroshima University, 大学院・先端物質科学研究科, 助教 (90452614)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | リン酸 / ポリリン酸 / ポリリン酸キナーゼ / 代謝 / ATP / phoU / Phoレギュロン / 発酵 |
|---|
| Research Abstract |
Inorganic polyphosphate (polyP) is a linear polymer of hundreds of phosphates linked by high-energy phosphoanhydride bonds. Since polyP is synthesized from ATP by polyP kinase (PPK), the regulation of polyP synthesis is thought to be closely related to the cellular metabolism and the energy status. Previously, we found that polyP accumulation stimulated by phoU mutation in Escherichia coli results in the changes in the expression of the genes involved in the several metabolic pathways. In this study, we analyzed the relationship between polyP accumulation and nitrate respiration (NR), one of the pathways found in the altered gene expression caused by phoU disruption in E. coli. The presence of a novel repression function of PhoU that regulates NR pathway besides Phoregulon was suggested from the analysis using a △phoU strain. The interaction of PPK to the proteins involved in TCA cycle and NADH production was revealed by pull-down analysis using PPK. These results suggest the direct relationship between polyP synthesis and the regulation of central metabolic pathway.
|
Research Products
(15 results)
