2011 Fiscal Year Final Research Report
Genome-wide screening of essential genes showing haplo-insufficiency in secretory protein production in yeast
| Project/Area Number |
21780076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
HOSHIDA Hisashi 山口大学, 大学院・医学系研究科, 准教授 (00314823)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 分泌性タンパク質生産 / 必須遺伝子 / ハプロ不全 / Saccharomyces cerevisiae |
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| Research Abstract |
To identify the genes affecting heterologous gene expression in Saccharomyces cerevisiae, heterozygous knockout strains of essential genes were used. As a result, 7 mutants that enhance expression of a secretory luciferase were identified. On the other hand none of knockout strain showed significantly lower expression except for weak-growth mutants. Six genes among the knockout strains were involved in RNA metabolism.
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