2011 Fiscal Year Final Research Report
Proteins involved in biosynthesis of sulfur-containing compounds
| Project/Area Number |
21780077
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kochi University |
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Principal Investigator |
KATO Shin-ichiro 高知大学, 教育研究部・総合科学系, 講師 (60346707)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 微生物酵素 / 含硫化合物 |
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| Research Abstract |
Cysteine desulfurase is a PLP-dependent enzyme and generates sulfur atom for the biosynthesis of sulfur-containing compounds, such as thiamine, biotin, and FeS cluster. We purified three cysteine desulfurases from obligate anaerobe, Clostridium acetobutylicum, and characterized their enzymatic properties. CAC2234 showed highest reactivity on L-Cys, we thus used them to examine the sulfur transfer from L-[^<35> S] Cys. We found five proteins specifically time-dependently labeled by^<35> S.
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