2011 Fiscal Year Final Research Report
Analysis of metabolism of substances within mitochondria of sake yeast during sake brewing
Project/Area Number |
21780078
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
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Research Institution | Saga University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Keywords | 清酒 / ミトコンドリア / 酵母 / 物質代謝 / 育種 / ピルビン酸 |
Research Abstract |
Since industrial alcoholic fermentation is performed without oxygen, the structure and the role of yeast mitochondria during industrial alcoholic fermentation have not been studied. We have, for the first time, elucidated the structure and role of yeast mitochondria during sake brewing. In order to investigate if yeast mitochondria can be targets of breeding, 29 deletants of mitochondrial transporters and 22 mutants overexpressing mitochondrial transporters were constructed and sake brewed with the mutants were analyzed. It was elucidated that substances of sake brewed with themutants were statistically significantly different from those of the parent strain, indicating that yeast mitochondrial transporters have a role in metabolism of substances during sake brewing. Based on the finding, sake yeast underproducing pyruvate was developed by isolating a sake yeast mutant resistant to an inhibitor of mitochondrial pyruvate transport, ethyl alpha-transcyanocinnamate. The developed strain exhibited pyruvate underproduction on a factors scale sake brewing.
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Research Products
(78 results)
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[Journal Article] Whole-Genome Sequencing of Sake Yeast Saccharomyces cerevisiae Kyokai no. 72011
Author(s)
T. Akao, I. Yashiro, A. Hosoyama, H. Kitagaki, H. Horikawa, D. Watanabe, R. Akada, Y. Ando, S. Harashima, T. Inoue, Y. Inoue, S. Kajiwara, K. Kitamoto, N. Kitamoto, O. Kobayashi, S. Kuhara, T. Masubuchi, H. Mizoguchi, Y. Nakao, A. Nakazato, M. Namise, T. Oba, T. Ogata, A. Ohta, M. Sato, S. Shibasaki, Y. Takatsume, S. Tanimoto, H. Tsuboi, A. Nishimura, K. Yoda, T. Ishikawa, K. Iwashita, N. Fujita, H. Shimoi
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Journal Title
DNA Research
Volume: 18(6)
Pages: 423-34
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[Journal Article] Breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport2010
Author(s)
Horie Kenta, Oba Takahiro, Motomura Saori, Isogai Atsuko, Yoshimura Takashi, Tsuge Keisuke, Koganemaru Kazuyoshi, Kobayashi Genta and Kitagaki Hiroshi
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 74 (4)
Pages: 843-847
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[Presentation] 酵母ミトコンドリアの機能は発酵から呼吸への転換を制御する2009
Author(s)
北垣浩志、Lauren Cowart, Nabil Matmati, David Montefusco, Jason Gandy, Silvin Vaena de Avalos, Sergei Novgorodov, Jim Zheng, Lina Obeid, Yusuf Hannun
Organizer
酵母ミトコンドリアの機能は発酵から呼吸への転換を制御する
Place of Presentation
名古屋大学
Year and Date
2009-09-24
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