2011 Fiscal Year Final Research Report
Analysis of a highly stable cholesterol oxidase from a gram-negative bacterium
| Project/Area Number |
21780082
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | コレステロール / コレステロールオキシダーゼ / 臨床検査 / Chromobacterium / グラム陰性細菌 |
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| Research Abstract |
Cholesterol oxidase is a valuable enzyme which is used for determination of cholesterol in human blood. We previously discovered a thermal, organic solvent and detergent-tolerant cholesterol oxidase from a gram-negative bacterium Chromobacterium sp. DS-1.In this study, the mechanism of the high stability of the enzyme was studied. As the result, it was found that a coenzyme FAD is involved in the stability of the enzyme.
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