2010 Fiscal Year Final Research Report
Combinatorial biosynthesis of plant triterpenoids in engineered yeasts
| Project/Area Number |
21780100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Osaka University (2010)
Yokohama City University (2009) |
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Principal Investigator |
SEKI Hikaru Osaka University, 工学研究科, 特任助教 (30392004)
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| Project Period (FY) |
2009 – 2010
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| Keywords | トリテルペノイド / 組換え酵母 / P450 |
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| Research Abstract |
Triterpenoids, with increasing evidence of their health beneficial properties, is a diverse group of secondary metabolites. Cytochrome P450 monooxygenases (P450s) play critical roles in site-specific oxidations of triterpene skeleton ; thereby create structural variation of triterpenoid aglycones. In this study, we identified several P450 enzymes involved in triterpenoid biosynthesis by using "homology-based" and "gene co-expression analysis" methods. These identified P450s were coexpressed with oxidosqualene cyclase, such as β-amyrin synthase, in engineered yeast to produce rare-natural or non-natural terpenoid products.
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[Journal Article] In vitro proliferation and triterpenoid characteristics of licorice (Glycyrrhiza uralensis Fischer, Leguminosae) stolons.2010
Author(s)
Kojoma, M., Ohyama, K., Seki, H., Hiraoka, Y., Asazu, S-N., Sawa, S., Sekizaki, H., Yoshida, S., Muranaka, T.
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Journal Title
Plant Biotechnology 27
Pages: 59-66
Peer Reviewed
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[Journal Article] Expressed sequence tags from rhizomes of Glycyrrhiza uralensis.2009
Author(s)
Sudo, H., Seki, H., Sakurai, N., Suzuki, H., Shibata, D., Toyoda, A., Totoki, Y., Sakaki, Y., Iida, O., Shibata, T., Kojoma, M., Muranaka, T., Saito, K.
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Journal Title
Plant Biotechnology 26
Pages: 105-107
Peer Reviewed
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[Presentation] Identification of cytochrome P450s involved in triterpenoid saponin biosynthesis in licorice.2010
Author(s)
Seki, H., Ohyama, K., Sawai, S., Mizutani, M., Ohnishi, T., Sudo, H., Akashi, T., Aoki, T., Saito, K., Muranaka, T.
Organizer
21th International Congress on Arabidopsis Research
Place of Presentation
Yokohama
Year and Date
2010-06-07
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