Establishment of new lipidomics approach

2011 Fiscal Year Final Research Report

• Project/Area Number: 21780110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tokyo Metropolitan University (2011); Hamamatsu University School of Medicine (2009-2010)
• Principal Investigator: INOUE Naoko 首都大学東京, 人間健康科学研究科, 特任助教 (00509515)
• Project Period (FY): 2009 – 2011
• Keywords: 分析化学 / 脂質

Research Abstract

We successfully established the new lipidomics approach named "TLC-Blot-MALDI imaging" which visualizes the lipid pattern obtained from thin layer chromatography by imaging mass spectrometry which is new analytical skills.
In this study, we evaluated the validity of the technique, and also applied to the biological samples. As a result, we succeeded to detect very small amounts of lipids which hardly detected by conventional methods. Moreover, comprehensive analyses were also performed to know the lipid dynamics change with molecular species level.

Research Products

• [Journal Article] (2012)
  • Author(s): Chansela P, Goto-Inoue N, Zaima N, Hayasaka T, Sroyraya M, Kornthong N, Engsusophon A, Tamtin M, Chaisri C, Sobhon P, Setou M
  • Journal Title: PLos One Volume: Vo. 7 Pages: e33154
• [Journal Article] (2012)
  • Author(s): Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL
  • Journal Title: Anal Bioanal Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Goto-Inoue N, Hayasaka T, Setou M
  • Journal Title: Methods Enzymol. Volume: Vo. 478 Pages: 287-301
• [Journal Article] (2011)
  • Author(s): Katagiri U Y, Sato B, Yamatoya K, Taki T, Goto-Inoue N., Setou M, Okita H, Fujimoto J, Ito T, Toshimori K, Kiyokawa N.
  • Journal Title: Biochem Biophys Res Commun Volume: Vo. 406 Pages: 326-331
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Valdes-Gonzalez, Goto-Inoue N, Hirano W, Ishiyama H, Hayasaka T, Setou M, Taki T
  • Journal Title: J Neurochem Volume: Vo. 116 Pages: 678-683
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Zaima N, Goto-Inoue N, Hayasaka T, Enomoto H, Setou M
  • Journal Title: Anal Bioanal Chem Volume: Vo. 400 Pages: 1865-1871
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Miyamura N, Nakamura T, Goto-Inoue N, S, Sakaida I, Setou M, Nishina H
  • Journal Title: Biochem Biophys Res Commun Volume: Vo. 408 Pages: 120-125
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Xu H, Kongmanas K, Kadunganattil S, Smith CE, Rupar T, Goto-Inoue N, Hermo L, Faull KF, Tanphaichitr N
  • Journal Title: J Lipid Res Volume: Vo. 52 Pages: 2187-2197
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Zaima N, Goto-Inoue N, Setou M
  • Journal Title: J Oleo Sci. Volume: Vo. 60 Pages: 93-98
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Goto-Inoue N, Hayasaka T, Taki T, Valdes Gonzalez T, Setou M
  • Journal Title: Journal of Chromatography A Volume: Vo. 1216 Pages: 7096-7101
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Goto-Inoue N, Hayasaka T, Zaima N, Setou M
  • Journal Title: Glycobiology Volume: Vo. 19 Pages: 950-957
  • Peer Reviewed
• [Presentation] イメージングマススペクトロメトリーによる皮膚臨床組織切片の解析 (2011)
  • Author(s): 井上菜穂子、内田良一、早坂孝宏、中島喜美子、佐野栄紀、瀬藤光利
  • Organizer: 日本医用マススペクトル学会
  • Year and Date: 20110000
• [Presentation] イメージングマススペクトロメトリーにおける金ナノ微粒子の応用 (2010)
  • Author(s): 井上菜穂子、早坂孝宏、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第1回アジア・オセアニア質量分析会議
  • Year and Date: 20100000
• [Presentation] Imaging mass spectrometry for glycomics (2010)
  • Author(s): 井上菜穂子
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Year and Date: 20100000
• [Presentation] イメージングマススペクトロメトリーによる糖脂質の局在解析 (2009)
  • Author(s): 井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
  • Organizer: 日本糖質学会
  • Year and Date: 20090000
• [Presentation] イメージングマススペクトロメトリーを用いたマウス精巣の脂質解析 (2009)
  • Author(s): 井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
  • Organizer: 日本質量分析学会
  • Year and Date: 20090000
• [Book] 質量顕微鏡法4. 7薄層クロマトグラフィー上でのイメージング(TLC-Blot-MALDIイメージング) (2009)
  • Author(s): 井上菜穂子、瀧孝雄
  • Publisher: シュプリンガー・ジャパン株式会社