2010 Fiscal Year Final Research Report
Molecular biological analysis of microbial community in decayed wood
| Project/Area Number |
21780164
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
YOSHIDA Makoto Tokyo University of Agriculture and Technology, 大学院・農学研究院, 特任准教授 (30447510)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 木材腐朽 / 菌類 |
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| Research Abstract |
Detection method of fungi existing in decayed wood was developed in the present study. Fungal community in decayed wood was analyzed by denaturing gradient gel electrophoresis following amplification of fungal ITS region by PCR. The analysis suggested the existence of various fungi in decayed wood. Moreover, visualization of mycelium by FISH was examined. As a result, FISH with DNA probe targeting ITS region of fungi was failed, while the analysis with PNA probe successfully visualized the mycelia of wood rotting fungi.
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