2010 Fiscal Year Final Research Report
A proteomic approach to cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis
| Project/Area Number |
21780166
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Okinawa Institute of Science and Technology (2010)
Kyoto University (2009) |
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Principal Investigator |
NAKASHIMA Keisuke Okinawa Institute of Science and Technology, マリンゲノミックスユニット, 研究員 (10422924)
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| Project Period (FY) |
2009 – 2010
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| Keywords | セルロース |
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| Research Abstract |
We report a western blotting system to assess cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis. Rabbit polyclonal antibodies were raised against several synthetic peptides corresponding to the parts of Ci-CesA, which is the cellulose synthase of C. intestinalis. After purifications with affinity columns using the peptides and an adsorption with Ciona acetone powder, either non-specific reactivity or cross-reactivity was reduced so that the single band was obtained with the estimated molecular mass of Ci-CesA, only in microsome preparations. For sample preparations, French-press worked fine for Ciona embryos.
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