2011 Fiscal Year Final Research Report

The establishment of germ-free fish in guppy Poecilia reticulates

Research Project

Project/Area Number	21780185
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General fisheries
Research Institution	Nihon University
Principal Investigator	MANO Nobuhiro 日本大学, 生物資源科学部, 講師 (10339286)
Project Period (FY)	2009 – 2011
Keywords	水産学 / 微生物 / 免疫学 / 仔稚魚
Research Abstract	Germ-free(GF) animals are animals that have no microorganisms or parasites living in or on it. In the present study, we established the GF fish(GFF) from ovoviviparity fish guppy Poecilia reticulate, and compared the body growth and lymphoid tissues of GFF and conventional fish(CF) groups. Rearing of GFF obtained was possible for over 100 days and the body growth differed in the two groups. GFF also have a reduced number of leucocytes distributed in the lamina propria of intestine and kidney tissues compared with CF. These results indicate that the CFF is an excellent tool for analyzing of the relationship between fish metabolism containing immune modulation and aquatic microorganism.

(1 results)