2010 Fiscal Year Final Research Report
The molecular mechanism of catch contraction based on the structural characteristics of twitchin of bivalve smooth muscle
| Project/Area Number |
21780195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Mie University |
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Principal Investigator |
FUNABARA Daisuke Mie University, 大学院・生物資源学研究科, 准教授 (00335150)
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| Project Period (FY) |
2009 – 2010
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| Keywords | キャッチ収縮 / 二枚貝 / ミオシン / アクチン / トゥイッチン / リン酸化 |
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| Research Abstract |
Myosin S1 of catch muscle of mussel Mytilus galloprovincialis was expressed using a protein expression system using the wheat germ extract. The myosin binding regions on the twitchin molecule were predicted with the ITC analysis using the peptides designed according to the sequence of twitchin and the myosin loop 2 peptide. The expression of myosin and paramyosin genes in catch muscles of mussel was suppressed by the RNA interference methods.
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Research Products
(5 results)
