2010 Fiscal Year Final Research Report
Identification and functional analysis of sugar chains involved in bone differentiation of mesenchymal stem cells, and bone regeneration
| Project/Area Number |
21780265
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Tottori University |
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Principal Investigator |
HOSAKA Yoshinao Tottori University, 農学部, 准教授 (00337023)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 間葉系幹細胞 / 骨分化 / コンドロイチン硫酸 |
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| Research Abstract |
In this study, we identified CS/DS sugar chain, which closely related to bone differentiation, and then analyzed its functions. Experiments using cultured cells, five types CS/DS (CS-A, -B, -C, -D and -E) chain were compared the ALP activity and calcium deposition and resulted CS-E had the strongest ability to induce bone differentiation. The CS-E-containing sponges (2ug/defect) were implanted in the site of bone defects created in Wistar rat skull, the bone regeneration was confirmed. The rate of bone regeneration in a 15-week experimental period was approximately 30% of defects.
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