Study on a regulation system of cellular functions in adiposities by sphingolipids.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780304
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Hokkaido University
• Principal Investigator: MITSUTAKE Susumu Hokkaido University, 大学院・先端生命科学研究院, 特任准教授 (10344475)
• Project Period (FY): 2009 – 2010
• Keywords: セラミド / スフィンゴミエリン / スフィンゴ脂質 / 糖尿病

Research Abstract

Sphingolipids has been demonstrated to be a lipid mediator in a variety of cellular events as a component of lipid microdomain. In this study, we found that the deficiency of SMS2, one of the sphingomyelin synthase, reduced the visceral fat of the mice. We further demonstrated that the physical interaction among SMS2, caveolin, and CD36 was important for the storage of neutral lipids.

Research Products

• [Journal Article] ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis. (2010)
  • Author(s): Ohno Y., Suto S., Yamanaka M., Mizutani Y., Mitsutake S., Igarashi Y., Sassa T., Kihara A.
  • Journal Title: Proc Natl Acad Sci USA 107(43) Pages: 18439-18444
  • Peer Reviewed
• [Journal Article] ABCA12 dysfunction causes a disorder in glucosylceramide accumulation during keratinocyte differentiation. (2010)
  • Author(s): Mitsutake S., Suzuki C., Akiyama M., Tsuji K., Yanagi T., Shimizu H., Igarashi Y.
  • Journal Title: J Dermatol Sci 60(2) Pages: 128-129
  • Peer Reviewed
• [Journal Article] Ceramide kinase is not essential but might act as an Ca2+-sensor for mast cell activation. (2010)
  • Author(s): Mitsutake S., Kumada H., Soga M., Hurue Y., Asanuma F., Nagira M., Deguchi M., Date T., Yokose U., Inagaki Y., Sugiura M., Kohama T., Igarashi Y.
  • Journal Title: Prostaglandins Other Lipid Mediat 93(3-4) Pages: 109-112
  • Peer Reviewed
• [Journal Article] Evaluation of synthetic sphingolipid analogs as ligands for peroxisome proliferator-activated receptors. (2009)
  • Author(s): Tsuji K., Satoh S., Mitsutake S., Murakami I., Park J.J., Li Q., Chang Y.T., Chung S.K., Igarashi Y.
  • Journal Title: Bioorg Med Chem Lett 19(6) Pages: 1643-1646
  • Peer Reviewed
• [Journal Article] 構造から読み解く中性セラミダーゼの触媒機構 (2009)
  • Author(s): 沖野望, 谷元洋, 光武進, 吉村征活, 合田初美, 伊東信
  • Journal Title: 細胞 41(5) Pages: 182-185
• [Journal Article] セラミド合成酵素とセラミドリン酸化酵素 (2009)
  • Author(s): 水谷有紀子, 光武進, 五十嵐靖之
  • Journal Title: 細胞 5(41) Pages: 174-177
• [Journal Article] Ceramide biosynthesis in keratinocyte and its role in skin function. (2009)
  • Author(s): Mizutani Y., Mitsutake S., Tsuji K., Kihara A., Igarashi Y.
  • Journal Title: Biochimie 91(6) Pages: 784-790
  • Peer Reviewed
• [Presentation] スフィンゴミエリン合成酵素2は、食餌誘導性脂肪肝形成に関与する (2010)
  • Author(s): 光武進、横田はづき、座間宏太、山下匡、岡崎俊朗、渡辺研、五十嵐靖之
  • Organizer: 第83回日本生化学会大会口頭発表3P-0122
  • Place of Presentation: 日神戸国際会議場(神戸)
  • Year and Date: 20101207-20101210
• [Presentation] セラミドキナーゼによるマスト細胞活性調節 (2010)
  • Author(s): 光武進、五十嵐靖之
  • Organizer: 第3回セラミド研究会学術集会
  • Place of Presentation: 日東京大学(東京)
  • Year and Date: 2010-11-12
• [Presentation] 中性脂質蓄積/代謝に置けるスフィンゴミエリン合成酵素2の役割り Involvementof sphingomyelin synthase2 in neutral lipidmetabolism. (2009)
  • Author(s): 光武進、横田はづき、座間宏太、山下匡、岡崎俊朗、渡辺研、五十嵐靖之
  • Organizer: 第82回日本生化学会大会ポスター発表2P-064
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 20091021-20091024
• [Remarks] ホームページ等
  • URL: http://biomem.pharm.hokudai.ac.jp/
• [Patent(Industrial Property Rights)] スフィンゴミエリン合成酵素SMS2の生理機能を応用したメタボリックシンドローム改善、又は改善薬のスクリーニング方法 (2009)
  • Inventor(s): 光武進、座間宏太、五十嵐靖之
  • Industrial Property Rights Holder: 国立大学法人北海道大学大学
  • Industrial Property Number: 特許,特願2009-219751
  • Filing Date: 2009-09-24