Investigation of biological meaning of nuclear localization in vertebrate CMP-Sia synthetase

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780308
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: FUJITA Akiko Nagoya University, 生物機能開発利用研究センター, 研究員 (60535003)
• Project Period (FY): 2009 – 2010
• Keywords: シアル酸 / CMP-シアル酸合成酵素 / 細胞内局在 / 生合成

Research Abstract

CMP-sialic acid synthetase (CSS) is a prerequisite enzyme in sialic acid biosynthesis and localizes in the nucleus. First, we showed that the protein expression of CSS was regulated by proteasome in NIH3T3 cells. Then, The knockdown of CSS in medaka (Oryzias latipes) embryo using morpholino antisense oligo technique lead to the fatal lethality.

Research Products

• [Presentation] Investigation of evolutionarily conserved CMP-sialic acid synthetases activity for Sia species from prochordate (2010)
  • Author(s): Fujita, A. ; Yasukawa, Y. ; Sato, C.; Kitajima, K.
  • Organizer: BMB2010(第83回日本生化学会大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Further characterization of the evolutionally conserved sialic acid recognition region of CMP-sialic acid synthetases (2010)
  • Author(s): A.Fujita, C.Sato, Y.Yasukawa, K.Kitajima
  • Organizer: Sialoglyco2010
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2010-08-22
• [Presentation] Is CMP-Sia Synthetase (CSS) a Sole Determinant of the Sialic Acid Species expressed on the Cell Surface? (2010)
  • Author(s): A.Fujita ; Y.Yasukawa, K.Shimano, S.Go, C.Sato, K.Kitajima
  • Organizer: The 25th International Carbohydrate Symposium
  • Place of Presentation: 東京
  • Year and Date: 2010-08-02
• [Presentation] Structural bases for the Sia substrate recognition of CMP-sialic acid synthetases (2009)
  • Author(s): Fujita, A., Sato, C., Yasukawa, Y., Munster-Kuhnel, A.K., Gerardy-Schahn, R., Kitajima, K.
  • Organizer: 20th International Symposium on Glycoconjugates
  • Place of Presentation: Perto Rico
  • Year and Date: 2009-12-03
• [Presentation] CMP-シアル酸合成酵素のシアル酸分子種認識および核局在決定領域の生物種間比較(優秀プレゼンテーション賞受賞) (2009)
  • Author(s): 藤田明子, 佐藤ちひろ, 安川裕子, 北島健
  • Organizer: 第82回生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] CMP-シアル酸合成酵素の基質認識および細胞内局在決定領域の生物種間比較 (2009)
  • Author(s): 藤田明子, 佐藤ちひろ, 安川裕子, 北島健
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 高山
  • Year and Date: 2009-09-11
• [Presentation] 原索動物由来CMP-シアル酸合成酵素の性質
  • Author(s): 藤田明子、安川裕子、佐藤ちひろ、澤田均、窪川かおる、 Guerardel Yann、北島健
  • Organizer: 日本農芸化学会2011年度大会、要旨集