2011 Fiscal Year Final Research Report
Functional analysis of small open reading frame in plants
| Project/Area Number |
21780315
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HIGUCHI Mieko 独立行政法人理化学研究所, 植物ゲノム機能研究グループ, 特別研究員 (40443014)
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| Project Period (FY) |
2009 – 2011
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| Keywords | short open reading frames / マイクロアレイ / シロイヌナズナ / 光誘導性遺伝子 |
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| Research Abstract |
To investigate function of sORFs in plants, I developed custom microarrays including sORFs and annotated genes in Arabidopsis to analyze gene expression profile. Microarray analysis was carried out in various tissue types. The expression of sORFs could be detected in various plant tissues. Full length cDNA of sORF was verified by RACE experiment. Next, promoter region and sORF without stop codon was fused to GUS gene and then expressed it in Arabidopsis. GUS signal was detected in transgenic plants, indicating that sORF was actually translated in plants. I selected sORFs based on expression, conservation, peptide hormone-like sequence to generate sORF overexpression Arabidopsis plants. Some overexpression plants showed visible phenotypes. Thus, I conclude that sORFs have roles in growth and developmental processes.
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