2010 Fiscal Year Final Research Report
Development of a multifunctional quantum dot for in vivo tracing of fluorescent labeled cells
| Project/Area Number |
21790035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
HIGUCHI Yuriko Kyoto University, 薬学研究科, 特定助教 (40402797)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 量子ドット / 幹細胞 / イメージング / デンドリマー / エンドソーム脱出 |
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| Research Abstract |
Information supplied from tissue distribution and dynamics of transplanted cells decides the efficiency of cell therapy. Quantum dots (QDs) are useful for labeling cells because of their high fluorescence intensity. In this study, we developed PAMAM dendrimer-conjugated QDs, which could enhance cellular uptake of QDs ; therefore, cells were efficiently labeled by PAMAM dendrimer-conjugated QDs. Moreover, PAMAM conjugation could enhance endosomal escape of QDs, consequently fluorescent intensity in cells could be retained longer.
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