2010 Fiscal Year Final Research Report
Exploration of biosurfactant to develop high efficiency and safety vector for gene transfection
| Project/Area Number |
21790047
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Aichi Gakuin University |
|---|
Principal Investigator |
INOH Yoshikazu Aichi Gakuin University, 薬学部, 講師 (90434547)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | 遺伝子治療 / 遺伝子導入 / 非ウイルスベクター / 正電荷リポソーム / バイオサーファクタントクタント |
|---|
| Research Abstract |
An unsaturated hydrocarbon chain in phospholipid was reported to affect a phase transition and a fusogenic activity after mixing membranes, and consequently to achieve a high DNA transfection efficiency. We previously showed that a biosurfactant mannosylerythritol lipid-A (MEL-A) enhances the gene transfection efficiency of cationic liposomes. Here, we have studied the effects of unsaturated fatty acid ratio of MEL-A on the gene transfer efficiency by cationic liposomes using MEL-A with different unsaturated fatty acid ratios. As a result, we demonstrated that the MEL-A unsaturated fatty acid ratio strongly affects transfection efficiency.
|
